动物造模,2型糖尿病模型 z-bio 2022-11-02 368

　　Objective To explore the preparation method of diabetes rat model induced by high-fat diet combined with streptozotocin, and provide a stable diabetes animal model for clinical research.

　　Methods Thirty male SD rats were randomly divided into two groups: the normal control group (NG, n=10) was fed with normal diet; Type 2 diabetes group (DM, n=20) was fed with high fat diet (HFD) for 12 weeks and STZ 25 mg/kg was injected into tail vein. During the experiment, the weight of rats was measured every week. One week before STZ injection, the oral glucose tolerance test (OGTT), insulin tolerance test (ITT) and the area change under the blood glucose curve were observed. After STZ injection, observe the general conditions of rats (food intake, water consumption, hair luster, moisture and mental status of bedding), and randomly detect the blood glucose and fructose of rats.

　　Results Compared with NG group, the weight of DM group rats increased significantly after 12 weeks of feeding (P<0.05). In OGTT experiment, the blood glucose at 60, 90 and 120 min was significantly higher than that in NG group (P<0.05), and the area under the curve was significantly increased (P<0.05); In the IPITT test, the blood glucose of DM group was significantly higher than that of NG group (P<0.05), and the area under the curve was significantly increased (P<0.05). After STZ intervention, there was significant difference between the two groups in feeding, drinking and body weight (P<0.05). Randomized blood glucose and fructosamine in DM group were significantly higher than those in NG group (P<0.05).

　　Conclusion High fat fed rats caused insulin resistance and abnormal glucose tolerance after 12 weeks. Combined with low dose STZ, the type 2 diabetes rat model with stable blood glucose is an ideal animal model for the study of type 2 diabetes.