动物造模,过敏性结膜炎小鼠模型 z-bio 2022-11-02 365

　　Objective To establish allergic conjunctivitis mouse models under different antigens, different excitation pathways and different mouse weeks, and to screen the best sensitization conditions by comparing the immune response status, so as to provide new ideas and methods for the establishment of allergic conjunctivitis mouse models.

　　Methods 65 SPF Balb/c mice were used to establish allergic conjunctivitis model under the conditions of different antigens (ovalbumin, ragweed pollen, house dust mite), different ways of stimulation (gavage, aerosol inhalation, subcutaneous injection), and different weeks of age (1 week, 2 weeks, 3 weeks, 8 weeks). The relative expression levels of CCL5 mRNA and IL-17 mRNA in mouse conjunctiva tissues were detected by RT qPCR, and the percentage of IL-17 in CD4+T cells in spleen single cell suspension was detected by flow cytometry.

　　Results Compared with the negative control group, the percentage of IL-17, the expression of CCL5 mRNA and IL-17 mRNA in ovalbumin group, house dust mite group and ragweed pollen group increased, among which ragweed pollen group increased most significantly. Compared with the negative control group, the percentage of IL-17 and the expression of IL-17 mRNA in the subcutaneous injection group, gastric perfusion group, aerosol inhalation group and positive control group increased. The expression of CCL5 mRNA in the gastric perfusion group was close to that in the negative control group, and the expression level in the subcutaneous injection group was closer to that in the positive control group. The percentage of IL-17, the expression level of CCL5 mRNA and IL-17 mRNA in mice at 1 week and 2 weeks of age were similar, but significantly lower than those in mice at 3 and 8 weeks of age.

　　Conclusion ragweed pollen has better sensitization in the establishment of allergic conjunctivitis mouse model. The subcutaneous injection of challenge antigen can achieve satisfactory results in the construction of allergic conjunctivitis mouse model. Although there is no obvious eye symptoms and signs, the whole body and local areas are sensitized. The immune response of 3-week-old Balb/c mice was close to that of adult Balb/c mice.