动物造模,重度创伤失血性休克模型 z-bio 2022-11-02 349

　　Objective To establish a modified model of severe traumatic hemorrhagic shock in rats and investigate the protective effect of anesthesia on the establishment of STHS rat model.

　　Methods Forty adult healthy male SD rats were randomly divided into four groups: the normal group was divided into conscious control group (CC, n=10), the anesthetic control group (AC, n=10), and the trauma huck group were made into severe traumatic hemorrhagic shock rat models with abdominal open wounds combined with anesthesia or 50% of regular and quantitative controlled blood loss under conscious state after placing tubes in bilateral femoral arteries and left femoral veins under anesthesia, They were respectively conscious traumatic hemorrhagic shock group (CTHS, n=10) and anesthetic traumatic hemorrhagic shock group (ATHS, n=10). Observe the general physiological conditions of rats in each group during the experiment, and continuously monitor the hemodynamic indexes of rats in the experiment: mean arterial pressure (MAP), heart rate (HR), maximum rate of rise/fall of left ventricular pressure (± dp/dt max), arterial blood gas indexes: pH value, partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), blood red egg concentration (ctHb), lactic acid (Lac), blood glucose (Glu), bicarbonate concentration (cHCO3 -), alkali surplus (BE); At the end of the experiment, HE staining microscope was used to observe the damage of the rat heart, liver, lung and kidney.

　　Results The changes of HR and ± dp/dt max with time in CTHS and ATHS groups were significantly different (P<0.01). HR and ± dp/dt max in ATHS group decreased, while HR in CTHS group decreased (T1) and then increased or stabilized (T2, T3, T4, T5, T6); After shock, MAP in the ATHS group showed a gradual upward trend and finally returned to the normal level of 25%~40%, while in the CTHS group, MAP decreased rapidly after the compensatory growth period. PH, PaCO2, ctHb, cHCO3 -, BE in the CTHS and ATHS groups showed a gradual downward trend. Except for PH and ctHb, the decline in the CTHS group was significantly greater than that in the ATHS group (P<0.01); Glu, Lac, PaO2 showed a gradual upward trend, and the increase amplitude of CTHS group was significantly higher than that of ATHS group (P<0.01). Compared with CTHS group, the degree of liver, heart, lung and kidney injury in ATHS group was significantly reduced.

　　Conclusion Anesthesia plays a protective role in the establishment of severe traumatic hemorrhagic shock in rats. It can improve hemodynamics, reduce the accumulation of respiration and metabolites, and reduce the degree of organ damage.