动物实验,帕金森 z-bo 2020-08-21 487

　　Objective: To observe the effect of Ginkgo biloba extract (GBE) on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced by Parkinson's disease (PD) at different ages.

　　Method: 60 C57BL/6 mice were randomly divided into negative group, model group, high, medium, and low dose GBE group and positive group at the 8th and 32nd week, and the PD was prepared by intraperitoneal injection of MPTP The animal model was treated with Ginkgo biloba extract. Climbing, suspension and swimming experiments are used to detect the motor function of the limbs of mice, and to monitor the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde in the brain tissue of mice. Aldehyde activity. Determined by photometry. The content of aldehyde (MDA) was determined by immunohistochemistry, and the number of positive cells of substantia nigra tyrosine hydroxylase (TH) and striatal dopamine transporter (DAT) were observed.

　　Results: (1) Compared with the negative control group, in the 8-week-old model group, motor function scores, brain GSH-Px activity, substantia nigra TH positive cells and striatal DAT positive cells were significantly reduced. done. The content of MDA in brain tissue increased significantly, indicating that the PD model has been successfully replicated. Compared with the model group, the motor function score, SOD and GSH-Px activity and the average optical density of the substantia nigra of TH-positive cells in the 8-week-old mice of the Ginkgo biloba extract group increased significantly, but the MDA content increased Up. Has been greatly reduced. (2) Compared with mice in the 32-week-old model group, the motor function scores, the motor function scores of substantia nigra TH-positive cells and striatal DAT-positive cells, the average optical density were significantly reduced, SOD and GSH-Px activities decreased and the brain Increased tissue MDA content indicates that the model has been successfully replicated. Compared with the model group, 32-week-old mice treated with Ginkgo biloba extract had significantly increased motor function scores, SOD activity, substantia nigra TH and striatum DAT positive cells, and increased brain MDA content. It has been intentionally reduced.

　　Conclusion: Ginkgo biloba extract has a certain protective effect on PD mice of various ages caused by MPTP.