动物造模,2型糖尿病小鼠模型 z-bio 2022-11-02 353

　　Objective To observe the changes of blood glucose in type 2 diabetes mouse model, and explore the feasibility of blood glucose 2 hours after meal as a blood glucose intervention evaluation point.

　　Methods Healthy C57BL/6 mice were selected as the experimental group of type 2 diabetes (T2DM) induced by high-fat diet plus streptozotocin (STZ), and the normal control group was set up. The continuous fasting blood glucose and continuous random blood glucose of the two groups were measured respectively, the blood glucose rule of the mice was analyzed, and the 2h postprandial blood glucose was evaluated as a scientific evaluation point for blood glucose intervention in T2DM mice; The stability of the evaluation point of 2-hour postprandial blood glucose intervention was verified by long-term observation of 2-hour postprandial blood glucose fluctuations in diabetes mice.

　　Results The continuous fasting blood glucose of T2DM mice showed that the blood glucose change was relatively stable during 2-3 hours after meals. After fasting for more than 4 hours, the blood glucose of T2DM mice continued to decrease significantly, while that of the normal group increased slowly, and the blood glucose remained relatively stable; Within 7 weeks, the blood glucose fluctuation of T2DM mice at 2h after meal showed that the blood glucose was stable at 2h after meal.

　　Conclusion The 2h postprandial blood glucose can stably reflect the changes of blood glucose in T2DM mice model, which has a good evaluation value of blood glucose intervention experiment.