动物造模,人源化小鼠模型 z-bio 2022-11-02 268

　　Purpose: To evaluate TNF ⁃ α Susceptibility of host to listeria monocytogenes infection after intervention of monoclonal antibody.

　　Methods: The animals were divided into C57BL/6 mice infected with TNF α Humanized mouse infection control group, TNF ⁃ α The humanized mouse adalimumab intervention group (n=6). The control group mice were injected with 200 normal saline 24 hours before infection μ 50. The mice in the adalimumab group received intravenous injection of adalimumab 200 at the dose of 10mg/kg 24 hours before infection μ Ｌ。 Infection by intraperitoneal inoculation of Listeria monocytogenes 1 × 104CFU was used to detect the amount of bacteria, pathological changes and distribution of immune cells in liver and spleen tissues, and the difference between the control group and the antibody intervention group was statistically analyzed.

　　Results: After 4 days of infection with Listeria monocytogenes, the area of microabscesses in the liver of the mice in the antibody intervention group was significantly larger than that of the control group (P.

　　Conclusion: TNF ⁃ α It plays an important role in host immunity against listeria monocytogenes α After mcab intervention, the host disease progression was significantly aggravated.