动物造模,动物模型 z-bio 2022-11-02 279

　　Objective To establish an animal model of KM mice with low immunity, and to identify the establishment of the animal model by a simple and rapid method.

　　Methods Cyclophosphamide (100mg/kg), hydrocortisone (80mg/kg), 60% ethanol solution (20mL/kg) and UVA ultraviolet skin irradiation (long wave) (10J/cm2) were administered orally for three consecutive days. The body weights of mice before and after the test were measured, and the IgG, IgA The content of IgM, the content of CD19 of B lymphocytes and CD3 of T lymphocytes in peripheral blood of mice were measured by flow cytometry, and the situation of peripheral blood cells of mice was analyzed by automatic blood analyzer.

　　Results The immunosuppressive model of KM mice in cyclophosphamide group and hydrocortisone group was established, but the immunosuppressive model of 60% ethanol group and ultraviolet radiation group was not established. Further experiments were needed to explore, measure the weight of mice, detect the content of IgG, IgA, IgM in mouse serum, and analyze the peripheral blood B, T lymphocytes and blood routine of mice, It can be used as a reference test index for the establishment of KM mouse model with low immunity.

　　Conclusion The animal model of KM mice with low immunity established by continuous oral administration of cyclophosphamide (3d100mg/Kg) is established. From the simplicity of various detection indicators, body weight, serum immunoglobulin content, and blood routine examination are simple and easy to obtain, with low detection cost, short time cycle, and high detection efficiency, which can be used as indicators for establishing and confirming animal models in daily detection work.