动物造模,肝癌模型 z-bio 2022-11-02 332

　　Objective: To explore the mechanism of hepatocarcinogenesis by establishing a C57BL/6 mouse model of chemically induced liver cancer to simulate the development of liver cancer.

　　Methods: 110 C57BL/6 mice were randomly divided into the control group (30 mice) and the model group (80 mice). The mice in the control group were not treated. The model group was established by intragastric administration of CCl4 combined with intraperitoneal injection of DEN; The weight of mice was measured continuously. The morphology of liver was observed at different time points, the levels of liver function related enzymes in venous blood serum were measured, and the pathological changes of liver tissue were observed.

　　Results: Compared with the control group, the weight of the mice in the model group decreased gradually, the liver morphology was abnormal, the serum liver function related enzymes ALT and AST were increased, and there were significant histopathological changes in liver cancer.

　　Conclusion: Diethylnitrosamine (DEN) combined with carbon tetrachloride (CCl4) can induce liver cancer in C57BL/6 mice, providing a reliable animal model for studying the molecular mechanism of liver cancer.