动物造模,胸主动脉瘤/夹层模型 z-bio 2022-11-02 305

　　Objective: To explore a simple and rapid method to evaluate the success of the establishment of the mouse model of thoracic aortic aneurysm/dissection.

　　Methods: Twenty four three week old male C57BL/6 mice were randomly divided into model group and control group. Mice in the model group were given normal diet and dissolved β- Aminopropionitrile (BAPN) was fed with drinking water for 4 weeks; The control group was given normal diet and normal drinking water. The body weight of mice was dynamically monitored, and the thoracic aorta and left common carotid artery of mice were detected by Vevo2100 small animal ultrasound at the fourth week of modeling, and the thoracic aorta of mice was observed by Micro CT.

　　Results: Compared with the control group, the weight of mice in the model group decreased significantly at the 4th week of modeling; Ultrasound and Micro CT examination of small animals showed that the thoracic aorta vessels in the model group were significantly expanded and tumorigenic; The ultrasonic model group of small animals can detect the change of pulse wave velocity, and the degree of arteriosclerosis is significantly improved.

　　Conclusion: Small animal ultrasound can simply, quickly and safely evaluate whether the establishment of mouse thoracic aortic aneurysm/dissection model is successful, dynamically observe the changes in the size of thoracic aortic aneurysm/dissection, and dynamically observe the degree of arteriosclerosis in mice.