动物造模,子痫前期大鼠模型 z-bio 2022-11-02 274

　　Objective: To explore the method of establishing preeclampsia rat model.

　　Methods: 39 SPF pregnant Wistar rats, including 16 cases in the reduced uterine blood perfusion (RUPP) group, 10 cases in the RUPP+irrigation group, and 13 cases in the sham operation group. RUPP operation or sham operation was performed on the 14th day of pregnancy respectively, and blood pressure, urine protein and the state of offspring were monitored on the 18th day of pregnancy.

　　Results: (1) The effects of RUPP operation on pregnant rats: Compared with sham operation group, the systolic blood pressure, diastolic blood pressure and mean arterial pressure of pregnant rats in RUPP group and RUPP+irrigation group increased to varying degrees, but the blood pressure in RUPP group increased unstably, and the diastolic blood pressure had no significant difference with sham operation group; The blood pressure of RUPP+irrigation group increased steadily, and was significantly higher than that of the simple RUPP group; Compared with sham operation group, RUPP group had significantly higher urine protein quantity at 18d and 24h of pregnancy. (2) The effect of RUPP operation on offspring: RUPP operation significantly reduced the live birth rate and the rate of offspring on the 20th day of pregnancy; The fetal weight and wet weight of placenta in RUPP group were significantly lower than those in sham operation group, especially in RUPP+irrigation group.

　　Conclusion: RUPP+irrigation method can induce stable PE clinical manifestations in rats, and it is recommended to be an improved RUPP modeling method.