动物模型,糖脂代谢紊乱 z-bo 2020-08-21 704

　　Objective: To establish a practical animal model of chronic metabolic disease, to explore the relationship between hyperuricemia and glucose and lipid metabolism, and the mechanism of its pathological damage.

　　Method: Combine a high-fat diet with a high-hypoxanthine diet + oxazine potassium to establish a model for detecting the dynamic changes of quail serum uric acid and triglycerides, and determine the serum-related indicators after the experiment. The aorta undergoes pathological examination.

　　Result: High-fat, high-hypoxanthine diet + oxazine potassium can significantly increase the serum uric acid and triglyceride content of quail. Glucose tolerance experiments showed that compared with other groups, the combined model had higher peak blood glucose levels, larger area under the blood glucose curve, and significantly higher insulin levels. The combination module can also increase the activity of serum aspartate aminotransferase, alanine aminotransferase, urea nitrogen and creatinine, and has obvious damaging effects on the liver, aorta and kidney. Bezafibrate and febuxostat can alleviate these harmful effects to varying degrees.

　　Conclusion: The co-introduction of high-fat, high-hypoxanthine diet and potassium oxalate can establish a simple and stable long-term animal model of chronic metabolic diseases.