动物造模,精神分裂症模型 z-bio 2022-11-03 216

　　Objective: To establish a rat model of schizophrenia, observe the pathological damage and the expression of PKA and endothelial chemoattractant factor-5 in prefrontal cortex, clarify the mechanism of nerve injury, and provide reference for clinical treatment

　　Methods: The experiment was randomly divided into two groups: the control group (normal feeding rats, subcutaneous injection of normal saline, n=15) and the induction group (intraperitoneal injection of amphetamine 0.5 mg/kg daily, to establish a model of schizophrenia, n=15). Hematoxylin eosin staining was used to detect the neuronal nucleus pyknosis in the prefrontal lobe of rats; The model of schizophrenia in rats was detected by the Sam Dodd stereotyped behavior scoring system and open field test; The expression of PKA and CCL5m RNA was detected by RT qRCR; The levels of PKA and CCL5 protein in experimental rats were detected by ELISA kit; Detection of IL-1 by Western blot α､ IL-1 β And IL-17 protein expression level

　　Results: Compared with the control group (0.85 ± 0.14), the stereotype score of rats in the induction group (2.38 ± 0.26) was higher, and that in the open field experiment (326.58 ± 15.47) was higher than that in the control group (198.55 ± 12.58) (P<0.05); Compared with the control group, the expression of PKA and CCL5 mRNA in the induced group increased (P<0.05); The contents of PKA (4.21 ± 1.05) mmol/g and CCL5 (3.76 ± 0.51) mmol/g in the induction group were higher than those in the control group (2.46 ± 0.67) mmol/g and (1.35 ± 0.24) mmol/g (P<0.05); Interleukin proinflammatory factor (IL-1 α､ IL-1 β､ The protein expression of IL-17) in the induced group (2.85 ± 0.35), (2.15 ± 0.27), (2.16 ± 0.32) was higher than that in the control group (1.02 ± 0.17), (0.94 ± 0.13), (1.05 ± 0.25) (P<0.05); The positive rate of cells in the induction group was higher than that in the control group. There were a large number of nucleus pyknosis and eosinophilic staining in the prefrontal vertebral cells (P<0.05)

　　Conclusion: The expression of PKA and CCL5 in the prefrontal cortex of schizophrenic rat model increases, inducing cognitive impairment