动物造模,单侧脑损伤模型 z-bio 2022-11-03 191

　　Objective: To evaluate the functional changes of brain regions related to brain perception and motor behavior response, which is an important strategy for clinical transformation research of major brain diseases based on non-human primates in brain science research, and has an important meaning for analyzing the regulation mechanism of brain functional loop under the physiological conditions

　　Methods: Male cynomolgus monkeys aged 7 to 9 years old were selected to construct Parkinson's model induced by right internal carotid artery injection of MPTP (1-methyl-4-phenyl-6-tetrahydropyridine) and ischemic stroke model caused by left middle cerebral artery occlusion. The effects of unilateral brain area injury on contralateral perception and motor function were evaluated using self-made mountain and valley ladder task device

　　Results: Normal monkeys were able to achieve full marks in the mountain ladder task and valley ladder task for 10 consecutive days; After the monkey Parkinson's model was established by injecting MPTP into the right internal carotid artery, PET-CT image analysis showed that the three experimental monkeys all had the damage of dopaminergic neurons in the right substantia nigra. In the mountain step task and valley step task, two experimental monkeys had significant damage to left hand motor function, and their spatial recognition ability was basically normal, while one experimental monkey not only had left hand motor ability damage, but also had abnormal spatial recognition ability or right hand motor ability damage; The monkey model of ischemic stroke caused by left middle cerebral artery occlusion was constructed by electrocoagulation. The MRI impact analysis showed that the right brain of the experimental monkey had typical ischemic infarct focus. In the mountain step task and valley step task, the experimental monkey had right hand motor function damage, but the spatial recognition ability was basically normal

　　Conclusion: The mountain and valley ladder task experiment can be effectively applied to the motor perception behavior evaluation of the animal model of unilateral brain injury in experimental monkeys