动物造模,动脉粥样硬化病证结合 z-bio 2022-11-03 227

　　Objective: To construct a rat model of atherosclerosis combining disease and syndrome, and provide an evaluation tool for the pharmacodynamic study of corresponding Chinese herbal compound

　　Methods: Referring to the relevant data and literature research, the diagnostic and therapeutic indexes for evaluating the rat model of atherosclerosis (Qi stagnation and blood stasis syndrome) were selected and put forward, and simvastatin tablets and Tongmai granules were used as the anti syndrome drugs. Combined with the modern research on the etiology and pathogenesis of atherosclerosis, It is proposed to use ice water bath, intragastric fat emulsion and tail vein injection of bovine serum protein to prepare a combined disease syndrome model of atherosclerosis (Qi stagnation and blood stasis syndrome) rats. During the experiment, the symptoms and signs of rats in each group are investigated and scored. After 8 weeks of modeling, the four levels of blood lipid and blood coagulation of rats in each group are detected, and the pathological changes of the ascending aorta are pre tested. After successful modeling, the anti evidence drugs are used to intervene, The four levels of blood lipid and blood coagulation were measured, and the pathological changes of ascending aorta and liver tissue were detected

　　Results: After 21 weeks of modeling, compared with the normal group, the symptoms and signs of rats in the model group changed significantly, the blood lipid level and fibrinogen content increased significantly, and the time to activate partial prothrombin decreased significantly. Lipid deposition appeared under the intima of the ascending aorta, and hepatocytes appeared vacuole and degeneration, basically in line with the clinical manifestations of atherosclerosis (Qi stagnation and blood stasis syndrome). After drug intervention, compared with the model group, The blood lipid level and fibrinogen content of rats in the drug anti syndrome group decreased significantly, and no obvious abnormality was found in the intima of ascending aorta and liver tissue

　　Conclusion: The combined induction method of ice water bath, intragastric fat emulsion and tail vein injection of bovine serum protein for 21 weeks can establish a rat model simulating clinical atherosclerosis (Qi stagnation and blood stasis syndrome), and has the characteristics of high modeling rate, similar to clinical symptoms and signs, which will provide evaluation for the pharmacodynamic study of anti atherosclerosis (Qi stagnation and blood stasis syndrome) Chinese herbal compound