动物造模,人肝癌裸小鼠原位癌模型 z-bio 2022-11-03 177

　　Objective: To construct a tumor bearing nude mouse model of human hepatoma cell line in situ by using human hepatoma cell line (HCCLM3 Luc) stably expressing luciferase reporter gene

　　Methods: The fluorescence intensity expression of HCCLM3 Luc in different cell numbers was quantitatively analyzed with an in vivo imaging instrument, and the luciferase activity of each cell number was detected to explore the linear correlation between the amount of photons produced by HCCLM3 Luc luciferase and the number of tumor cells. HCCLM3 Luc tumor tissue was inoculated into the liver lobe in situ and HCCLM3 Luc cell suspension was injected into the tail vein to construct human hepatocellular carcinoma in situ tumor models in nude mice; To compare the feasibility of two methods to construct nude mice model of human liver cancer cell line in situ, and optimize the key factors in the model construction

　　Results: The number of HCCLM3 Luc cells was positively correlated (correlation coefficient R2=0.9989, linear equation: Y=1155.8X+1 × 106), and the average ROI value is about 140 photons/s per cell. The tumor formation rate in situ [(82.5 ± 7.2)%] in the group of liver lobe in situ inoculation is significantly higher than that of tail vein injection [(34.1 ± 13.2)%]

　　Conclusion: A nude mouse model of tumor in situ of bioluminescent human liver cancer cell line has been constructed in this project. This model can use a small animal imager to monitor the tumor growth in real time