动物实验,肾动脉栓塞 z-bo 2020-08-21 541

　　Objective: To establish a canine renal artery embolization model with microsphere embolization agent and evaluate the effects of unilateral renal artery embolization and local tissue observation in dogs.

　　Method: The experimental animals are eight beagles. Immediately after embolization, the experiment was divided into 4 week, 12 week and 26 week groups, each group containing 2 animals. Choose the self-control of microsphere embolization group and normal control group. First, the left renal artery was embolized with microsphere embolization, and after the embolization, the immediate changes were checked by renal angiography and DSA at the 4th, 12th and 26th weeks, and then the experimental animals were sacrificed. , Histopathological observation of the kidney.

　　Result: gross pathological observation: compare the size of the embolized kidney with the size of the contralateral kidney. At 4, 12, and 26 weeks after embolization, the kidneys became smaller and smaller. The longer the embolization time, the smaller the size. Obviously, the color of the embolized kidney becomes bright and uneven. The surface of the embolized kidney gradually becomes smooth and uneven, and the kidney capsule becomes difficult to separate. Optical microscope: 4 weeks, 12 weeks, and 26 weeks after embolization; microsphere filling can still be seen in the arcuate twigs, the arterial wall is vitreous, and the arterial fibrosis; the embolization time increases with the number, and the early renal tissue has many degenerative changes , Such as atrophy, degeneration, old kidney infarction and late tubular regeneration, granulation tissue formation and remodeling, and other pathological changes. There are many progressive changes in kidney tissue, for example. However, in the embolized kidney, there are still almost normal glomerular and tubular structures.

　　Conclusion: The microsphere embolization agent has obvious embolic effect on unilateral renal artery in dogs.