动物造模,抑郁模型 z-bio 2022-11-03 223

　　Objective: To establish the depression model of C57BL/6 mice and preliminarily explore the effect of high frequency sound wave in Mozart K448 sonata on improving the depression symptoms of C57BL/6 mice

　　Methods: 1) Establishment of chronic stress model: The mice were divided into blank group (n=10) and model group (n=36, The model mice were randomly and evenly divided into model control group (n=12), fluoxetine group (n=12) and music group (n=12), The tail suspension test (TST) and forced swimming test (FST) were conducted at week 1, 5 and 7 of the experiment. After the behavioral experiment at week 7, the brain tissue of mice was taken to prepare homogenate, and the content of brain-derived neurotrophic factor (BDNF) was determined by enzyme-linked immunosorbent assay (ELISA)

　　Results: (1) The CUMS mouse model was successfully constructed. At the fifth week, the tail suspension immobility time of mice in the model group was significantly increased (P<0.01), and the forced swimming immobility time was increased, with a significant difference (P<0.05). (2) Compared with the model control group, the tail suspension immobility time of mice in the fluoxetine group was significantly shortened, with a significant difference (P<0.01), and="" the="" forced="" swimming="" immobility="" time="" was="" with="" no="" significant="" difference="" p="">0.05); Compared with the model control group, the tail suspension immobility time in the music group was shortened, with a significant difference (P<0.05), and="" the="" immobility="" time="" in="" forced="" swimming="" test="" had="" no="" significant="" with="" difference="" p="">0.05). Compared with the blank group, the content of BDNF in brain tissue homogenate in the model control group was significantly reduced, with a significant difference (P<0.01); Compared with the model control group, the BDNF content in brain tissue homogenate in the fluoxetine group increased significantly (P<0.01), but="" there="" was="" no="" significant="" difference="" between="" the="" music="" group="" and="" model="" control="" p="">0.05)

　　Conclusion: High frequency acoustic wave of Mozart K448 sonata can optimize the therapeutic effect of mouse depression model to a certain extent