动物造模,恐惧记忆模型 z-bio 2022-11-03 305

　　Objective: To optimize the best experimental parameters for establishing the rat model of conditioned fear memory by using orthogonal test, and to find the best experimental conditions

　　Methods: The memory model of conditioned fear in rats was established by using Pavlov's principle of conditioned fear; The orthogonal experimental design with three factors and three levels was used to observe the expression of fear memory under different parameters by taking the numbness reaction time of rats 24 hours after the establishment of the model as an indicator. The best experimental conditions were determined. The conditional fear memory model was established under the optimal experimental conditions, and the fear memory retention was detected 24 hours, 1 week, 2 weeks, 4 weeks and 8 weeks later

　　Results: The results of visual analysis showed that the influence ability of each factor was sound intensity=number of cycles>electric shock intensity; The results of ANOVA showed that the number of cycles had a significant impact on the experimental results (P<0.05), but="" the="" effects="" of="" sound="" intensity="" and="" electric="" shock="" were="" not="" significant="" p="">0.05). The optimal experimental conditions were determined as follows: sound 75dB, electric shock 0.8mA, 15 cycles. At 24h and 1 week after the establishment of the model, the fear memory of rats remained good, with significant differences compared with the control group (P<0.001 and P<0.05); after="" the="" second="" p="">0.05)

　　Conclusion: This experiment has clarified the primary and secondary factors affecting the conditioned fear memory model of rats, optimized the experimental conditions, and provided experimental basis for model standardization, normalization and subsequent research