动物造模,小鼠模型 z-bio 2022-11-03 369

　　Objective: To establish aseptic APPswe/PS1 using the method of cesarean section purification Δ E9 (PAP) double transgenic mouse model and preliminary observation of plaque deposition in animal brain, providing a new animal model for studying the relationship between intestinal flora and Alzheimer's disease

　　Methods: Male heterozygous mice with positive PAP were selected to mate with C57 wild type female mice in a ratio of 1 ∶ 2. Pregnant female mice underwent cesarean section in an ultra clean worktable, and sterile ICR mice were used to replace milk. Sterility status was detected every month after the operation; PCR was used to detect the genotype of PAP offspring from cesarean section; Immunohistochemical method was used to quantitatively detect the changes of plaque in the brain of 9-month old PAP mice

　　Results: There were 12 cases of cesarean section and 66 offspring rats were obtained. The survival rates of cesarean section and weaning were 95.45% (63/66) and 95.24% (60/63) respectively. After purification, the sterility was qualified according to the national standard. The immunohistochemical results showed that the plaque in the hippocampus of 9-month old sterile PAP mice was less than that of SPF animals of the same month

　　Conclusion: The bacteria carried by PAP mice were removed by caesarean section purification technology, and the plaque in the brain of 9 month old sterile PAP mice was reduced