动物实验,足底电击应激 z-bo 2020-08-21 553

　　Objective: To observe the effect of the duration of electric shock stress on the soles of the anxiety experiment.

　　Method: According to body weight, adult male SD rats were randomly divided into a control group and a plantar electric shock group, with 12 rats in each group. By combining the plantar electric shock group and orphan reproduction, we established the inevitable plantar electric shock (0.8mA, electric shock 10 seconds, remaining 20 seconds, once a day, 5 minutes in total), which is a rat anxiety model. In order to evaluate the anxiety behavior of rats, maze and open experiments were performed 7 days and 14 days after plantar electric shock. \r\nResult: In the plantar electric shock group, anxiety-like behaviors increased 7 days and 14 days after the plantar electric shock, and the difference was statistically significant (P u003c0.01). Therefore, the total number of arm entry was significantly less than that of the control group, and athletic performance decreased. In the open experiment, the number of invasions to the middle and the stay time in the middle of the plantar electric shock group were lower than those of the control group (P \u003c u003c0.01), and the total movement distance, the number of posts and the number of corrections were lower than the control group. Less than the control group. , Significantly lower than the control group (P \u003cu\→ u0.01c0.01), and sports search activity decreased. Comparing the behavioral results of the 7-day and 14-day plantar shock groups, as the number of days in the model increased, the time ratio of closed arms and the number of closed arms decreased (P\u003c0.05). , You can see that the anxiety level is decreasing. In the open experiment, the total exercise distance, the number of standing times and the number of corrections were less than 7 days, the difference was significant (P\u003c0.05), and the exercise exploration behavior of rats was further reduced and the degree was reduced. And show depressive behavior. increase. Conclusion: The different time course of plantar electric shock stress can cause anxiety-like behavior in rats and affect their exercise seeking activities. As the number of modeling days increases, the behavior of rats may change from anxiety to depression. The time-lapse selection of the plantar electric shock anxiety model is 7 days better than 14 days. \r\nObjective: To observe the effect of the pressure duration of ankle electric shock on the elevated maze and anxiety experiment. Methods: Adult male SD rats were randomly divided into control group and plantar electric shock group according to their body weight, with 12 rats in each group. The plantar electric shock group combined with orphan rearing to establish a rat anxiety model, the inevitable plantar electric shock (0.8mA, electric shock 10 seconds, rest 20 seconds, a total of 5 minutes, once a day). Be given. To evaluate the anxiety behavior of rats, elevated maze and mouth opening experiments were performed 7 days and 14 days after the plantar electric shock.

　　Result: 7 days and 14 days after the plantar electric shock, anxiety-like behavior increased in the plantar electric shock group, the difference was statistically significant (P \u003c0.01), the total number of arm access was significantly less than the control group, and the exercise ability decreased. In the open experiment, the number of invasions to the middle part and the stay time in the middle part of the plantar electric shock group were lower than those of the control group (P\u003c0.01), and the total movement distance, the number of upright times and the number of modifications were lower than the control group. Significantly less than the control group (P\u003c0.01), the activities of exercise exploration decreased. Comparing the behavioral results of the 7-day and 14-day plantar shock groups, the time ratio of closed arms and the number of closed arms decreased with the increase of modeling days (P\u003c0.05). You can see that the anxiety level has decreased. In the open experiment, the total exercise distance, the number of standing times and the number of modification times were less than 7 days, and the difference was significant (P\u003c0.05), which further reduced the exercise exploration behavior of rats, and the degree is decreasing, indicating depressive behavior increase.

　　Conclusion: The different time courses of the plantar electric shock stress can cause anxiety-like behaviors in rats and affect their exercise exploration activities. With the increase of modeling days, the behavior of rats may change from anxiety to depression. The time course selection of the plantar electric shock anxiety model is 7 days better than 14 days.