动物造模,皮肤型狼疮小鼠模型 z-bio 2022-11-03 389

　　Objective: To investigate whether the aged mice with systemic lupus erythematosus (TC) can be used as a mouse model for the study of cutaneous lupus by observing and identifying the skin symptoms

　　Methods: Observe the age of TC mice with skin symptoms, compare the skin symptoms of mice of the same age with C57BL/6 mice, detect anti dsDNA antibody and anti ANA antibody in serum of mice by ELISA, and detect the pathological symptoms of mice skin by HE staining

　　Results: Two thirds of TC mice showed hair loss, skin ulceration and other symptoms. The lesions first occurred at 40 weeks of age. The titers of anti dsDNA antibody and anti ANA antibody in serum of lupus mice with skin lesions were significantly higher than those of C57BL/6 mice (P<0.05); HE staining results showed that the stratum corneum of lupus mice was missing, the continuity of epithelial layer was interrupted, and a large number of lymphocytes were infiltrating into the dermis

　　Conclusion: The lupus mice will have skin lesions after 40 weeks, which shows that the mice can be used as a disease model to study chronic cutaneous lupus