动物造模,内脏高敏感模型 z-bio 2022-11-03 260

　　Objective: To establish rat visceral hypersensitivity animal model by foot electric shock and evaluate its effectiveness and advantages

　　Methods: Female SD rats were randomly divided into normal control (NC) group, foot shock (FSS) group and water repellent stress (WAS) group; FSS group was placed in the shock box for 1h every day and given electric shock stimulation with the stimulation voltage of 40V and the stimulation frequency of 20 times/min for 1s each time for 5min; The WAS group was given water avoidance stress for 1h every day; The modeling period is 10 days. Observe and record the general situation and defecation of rats, detect visceral sensitivity with abdominal wall retraction reflex (AWR), detect serum CRF, ACTH, CORT, 5-HT concentration and colon tissue 5-HT concentration with ELISA

　　Results: (1) The visceral sensitivity of FSS group rats was significantly higher than that of WAS and NC groups (P<0.05), and there was no obvious pathological damage to the colon mucosa. (2) The concentrations of CRF, ACTH, CORT, 5-HT in serum and 5-HT in colon tissue of FSS group rats were significantly higher than that of WAS and NC groups (P<0.05). (3) The fecal volume of FSS group rats was significantly higher than that of WAS and NC groups (P<0.05), and the fecal water content was significantly higher than that of NC group (P<0.05), Moreover, the defecation volume and fecal water content of rats were stable

　　Conclusion: Foot electric shock modeling method can establish visceral hypersensitivity model in rats and is superior to water avoidance stress method