动物实验,人α干扰素,淋巴细胞 z-bo 2020-08-22 557

　　Objective: To investigate the regulatory effect of human interferon-α (IFN-α) gene transformation on the immune function of mice after oral administration of bifidobacteria.

　　Method: Construct the Escherichia coli-Bifidobacterium shuttle expression vector pBADX-hIFNα2b containing human IFN-α gene, and transform the IFN-α gene into bifidobacteria by electroporation, and induce hIFN by L-arabinose. The body of bacteria collected after expressing α2b was made into a viable bacterial suspension, and then the Balb/C mice were administered intragastrically. Experimental group (IFN) mice were treated with 0.2% L-arabinose transformed with bifidobacteria to induce hIFN-α2b expression and 0.1ml 1010/ml viable bacteria; negative control group (NC). Using the same amount and the same volume of empty vector pBADX, the live bifidobacteria were transformed by intragastric administration, and the empty control group (BC) was intragastrically administered an equal volume of normal saline. Eat the stomach every other day for two weeks. Flow cytometry is used to detect the lymphocyte subsets and ratios in mouse thymus, spleen and blood, and flow cytometry kits are used to detect the content of immune factors (such as IFN-γ and IL-4). For.

　　Results: The proportion of CD3 + CD8 + and CD4 + CD8 + cells in the mouse thymus in the IFN group was significantly higher than that in the NS group and BC group (P0.05).

　　Conclusion: Oral oral administration of bifidobacteria transformed with hIFN-α can promote the proliferation and maturation of mouse thymus and spleen lymphocytes, and increase blood Th1 cytokine levels.