动物造模,心肌细胞线粒体损伤大鼠模型 z-bio 2022-11-03 259

　　Objective: To observe the morphological changes of mitochondria and the production of reactive oxygen species (ROS) in cardiac myocytes induced by electric stimulation of bupivacaine, and to establish an ideal model of rat cardiac myocytes poisoned by bupivacaine

　　Methods: Myocardial cells of male SD rats were freshly isolated with Langendorff device. After counting the cells, they were transferred to the doff tube and randomly divided into four groups: DMEM standing group, DMEM electric stimulation group, bupivacaine standing group, bupivacaine electric stimulation group. The experiment was repeated five times. Mitochondria morphology of myocardial cells was observed with transmission electron microscope, and ROS production was measured with multi-functional microplate detector

　　Results: There was no significant difference in mitochondrial swelling and ROS production between DMEM electric stimulation group and DMEM static group (P>0.05); However, the swelling degree of mitochondria in the electric stimulation group of bupivacaine was significantly higher than that in the static bupivacaine group (P=0.000), and the ROS production was also significantly increased (P<0.05)

　　Conclusion: Myocardial cells contract rhythmically under electrical stimulation, which can better simulate the myocardial mitochondrial damage in clinical bupivacaine poisoning