动物造模,子宫内膜异位症模型 z-bio 2022-11-03 388

　　Objective: To investigate the therapeutic effect of GnRH-a plus different doses of estrogen on endometriosis and its influence on recurrence

　　Methods: Fifty one rats with endometriosis were divided into five groups: control group (n=10), which was injected with 1 mg/kg normal saline subcutaneously once; Study group: anti dose group I (low dose estrogen group n=10), anti dose group II (medium dose estrogen group n=10), anti dose group III (high dose estrogen group n=10), GnRH-a group (n=11). The study group was given leuprorelin 1mg/kg subcutaneous injection for one time, and the anti dose groups I, II and III were given different doses of estradiol valerate (25 μ g/kg､50 μ g/kg､100 μ G/kg). At the same time, GnRH-a group was given normal saline by gavage, 3mL/animal, once a day in each study group, for a total of 14 days. At the 4th and 7th weeks after administration, open the abdomen to explore, measure the volume of endometriosis, take the tissue of endometriosis, and detect TNF in endometriosis by immunohistochemistry- α､ The expression of StAR, the blood collection method of orbital medial canthus venous plexus, and the serum CA125 level were detected by enzyme-linked immunosorbent assay

　　Results: Before administration, there was no significant difference in the volume of ectopic lesions in rats of each group (P>0.05). At the fourth week, the study group was significantly smaller than the control group, with a significant difference (P<0.05); At the 7th week, the volume of rats in group Ⅱ and Ⅲ of anti addition group increased significantly compared with those in group Ⅰ and GnRH-a of anti addition group (P<0.05). before="" tnf="" in="" ectopic="" lesions="" of="" rats="" each="" group-="" there="" was="" no="" significant="" difference="" the="" expression="" star="" p="">0.05). At the 4th week, TNF in different lesions in the study group- α､ The expression of StAR was significantly lower than that in the control group (P<0.05); TNF in group Ⅱ and Ⅲ of anti treatment group is higher than that in group Ⅰ and GnRH-a of anti treatment group- α､ The expression of StAR was high with significant difference (P<0.05)

　　Conclusion: GnRH-a plus low-dose estrogen has a good effect on rat endometriosis model, and the reverse addition of medium and high-dose estrogen may induce TNF- α､ StAR promotes the recurrence of endometriosis in rats