动物造模,幼羊颅骨缺损模型 z-bio 2022-11-03 287

　　Objective: To detect the expression of Dlx2 gene in the skull tissue of the small tailed Han sheep skull defect model, and to explore its significance for the skull defect. Methods Ten 1-month old small tailed Han sheep were selected to establish the skull defect model, and the new skull tissue at the defect site and the native skull tissue near the defect site were sampled. Real time PCR and Western blot were used to detect the mRNA and protein expression level of Dlx2

　　Results: Compared with the original skull tissue adjacent to the defect site, the expression level of Dlx2 mRNA and protein in the newborn skull tissue at the defect site was significantly higher (P<0.05)

　　Conclusion: The increased expression of Dlx2 gene may have important significance for the repair of skull defects