动物造模,阿尔茨海默病模型 z-bio 2022-11-03 261

　　Objective: To analyze the differentially expressed genes in the prefrontal cortex of Alzheimer's disease (AD) model mice and reveal the pathogenesis of AD from the transcriptome level

　　Methods: This study randomly selected nine month old female APP swe/PS1 Δ Five E9 (PAP) model mice and five wild type C57BL/6J mice each, RNA from prefrontal cortex tissue was extracted and sequenced with Illumina HiSeq 3000. The edger software was used to analyze the significance of expression difference. The changes of gene expression in AD group and control group were analyzed, and qRT ⁃ PCR was used to verify the six key differentially expressed genes. Then cluster analysis, GO function enrichment analysis, KEGG pathway enrichment analysis were performed on the differentially expressed genes

　　Results: 224 differentially expressed genes were found between AD group and control group (P<0.05, logfc="">1.0), of which 205 genes were up-regulated and 19 genes were down regulated. The results of qRT PCR validation of 6 key genes were consistent with the trend of RNA Seq. The results of GO function enrichment analysis showed that these differentially expressed genes were related to immune response, inflammatory response, chemokine activity and IgG binding; The enrichment analysis of KEGG pathway shows that these genes are involved in phagocytosis, lysosome, Toll like receptor signal pathway, cytokine receptor interaction, NF ⁃ κ B signal pathway and other important biological pathways

　　Conclusion: The differential expression genes related to AD are obtained, which provides an experimental basis for the study of AD related mechanism and treatment in model mice