动物造模,高尿酸血症性肾损害小鼠模型 z-bio 2022-11-03 286

　　Objective: To establish a reasonable and stable hyperuricemic renal damage mouse model, and provide a pathological model for screening and studying drugs for the treatment of hyperuricemic nephropathy

　　Methods: Potassium oxazinate, hypoxanthine, adenine, ethambutol and yeast extract were used alone, in combination with two drugs or in combination with three drugs, Observe the changes of serum uric acid, urea nitrogen and creatinine levels, liver xanthine oxidase (XOD) and adenosine deaminase (ADA) activity, kidney pathology and body weight in the hyperuricemic kidney damage model of mice established by different modeling time, modeling dose and modeling method

　　Results: Compared with the normal group, the level of serum uric acid and urea nitrogen in the single modeling group with hypoxanthine and potassium oxazinate significantly increased (P<0.01). Renal tubules were seen in the renal cortex, and salt crystals were seen in the renal medulla; The serum uric acid level and urea nitrogen of mice in the 7-day group treated with hypoxanthine, ethambutol and potassium oxazinate were significantly increased (P<0.01), the XOD activity of liver was significantly decreased (P<0.05), and eosinophilic insoluble protein was found in the proximal convoluted tubule of kidney; Compared with the normal group, the serum uric acid, urea nitrogen and creatinine values of mice in the 14 day group combined with yeast extract and potassium oxazinate and the 14 day group combined with yeast extract, adenine and potassium oxazinate significantly increased (P<0.01). In the group combined with yeast extract and potassium oxazinate, epithelial cells of renal tubules fell off, and eosinophilic insoluble protein was found in the proximal convoluted tubules of the kidney, Salt crystals can be seen in the renal medulla of mice in the combination group of yeast extract, adenine and potassium oxazinate. The weight of mice in the combination group of yeast extract and potassium oxazinate increased faster than those in the combination group of yeast extract, adenine and potassium oxazinate, with a significant difference between the two groups (P<0.05)

　　Conclusion: Compared with other modeling methods, the model of hyperuricemia induced renal damage in mice established by yeast extract and potassium oxazinate is more stable and has no significant impact on the weight of mice. At the same time, this modeling method is more in line with clinical characteristics, so it is more appropriate to establish a hyperuricemia induced renal damage model in mice by yeast extract and potassium oxazinate for 14 days