动物造模,胃炎癌转化模型 z-bio 2022-11-03 270

　　Objective: To construct a rat model of gastritis and cancer transformation and explore new early serodiagnostic indicators of gastric cancer

　　Methods: N-methyl-n-nitro-nitro-n-nitrosoguanidine drinking water (MNNG) and high salt diet were used to induce the transformation model of gastritis and cancer in rats. Nuclear magnetic resonance imaging and histopathology were used to observe the process of gastric deterioration in rats. qRT PCR was used to detect the expression levels of miR-17-5p and miR-374-5p in serum of rats at different time points (weeks 0,124,36,48)

　　Results: MNNG drinking water combined with high salt diet successfully induced the transformation of gastritis and cancer in rats, and went through the process of atrophic gastritis (the 24th week of modeling) and atypical hyperplasia (the 48th week of modeling). The expression levels of miR-17-5p and miR-374-5p in serum were positively correlated with the degree of gastric deterioration in rats. Compared with the normal control group, at the 36th and 48th weeks, the expression levels of miR-17-5p increased about 8 times (t=4.1 P<0.001) and 10 times (t=5.3 P<0.001) respectively; At the 48th week, the expression level of miR-374-5p increased about 6 times (t=3.6 0.001<P<0.002)

　　Conclusion: The rat gastritis cancer transformation model constructed by MNNG drinking water and high salt diet provides a good animal model for studying the pathogenesis of gastric cancer. Serum miR-17-5p and miR-374-5p are potential non-invasive diagnostic indicators for early gastric cancer