动物造模,脓毒症模型 z-bio 2022-11-04 249

　　Objective: To investigate the changes of coagulation function in sepsis rat model induced by cecal ligation and puncture

　　Methods: CLP was used to induce sepsis in SD rats. At 8h, 16h and 48h after operation, relevant coagulation indexes were detected, and the histopathological changes of lung, kidney, liver and spleen were observed with HE staining

　　Results: The 12 day survival rate of CLP induced sepsis rats was 30%, and the morbidity was acute and the mortality was high. In the acute stage of disease development, the APTT time of CLP rats was prolonged at 8h (P<0.05), and the PT time was prolonged at 16h (P<0.05), The XII activity of endogenous coagulation pathway and the VII activity of exogenous coagulation pathway were temporarily inhibited. TT was prolonged at 48h (P<0.01). The content of FIB gradually increased from 16h (P<0.001). The number of PLT in other important indicators related to coagulation and anticoagulation function decreased gradually from 8h (P<0.01); VWF: Ag content increased gradually from 8h (P<0.001); The amount of D ⁃ D increased gradually from 16h (P<0.05); PS: Ag content decreased significantly until 48h (P<0.001); The content of AT ⁃ III did not change significantly. Pathological examination found that lung, kidney, liver and spleen tissues were damaged to varying degrees, but no obvious venous thrombosis and bleeding characteristics were shown

　　Conclusion: The rat sepsis model has coagulation disorder in the acute phase, but no histopathological changes caused by coagulation function are observed