动物造模,全子宫切除模型 z-bio 2022-11-04 270

　　Objective To establish an experimental animal model of total hysterectomy in BALB/c mice, and to explore and analyze the characteristics and formation mechanism of the model

　　Methods The rats were divided into blank control group, sham operation group and model group; The model group received abdominal hysterectomy; In the sham operation group, only the skin was cut and the uterus was exposed. After the operation, the mice in each group were routinely fed, and the weight changes were dynamically observed. The content of estradiol in the peripheral blood and the histological changes of the ovary were detected at the end of the 4th week

　　Results All the mice in each group survived after operation, with good general symptoms, no obvious inflammation in the incision, and complete healing; There was no significant difference in the weight of experimental mice in each group before operation. At the 2nd, 3rd and 4th weekend after operation, the weight of mice in the model group was higher than that in the control group and sham operation group (P<0.05); The content of estradiol in peripheral blood of mice in model group was significantly lower than that in control group and sham operation group (P<0.01); No abnormality was found in the ovarian histology of mice in the blank control group and sham operation group, and abnormal histological changes such as irregular follicles, loose granular cells and apoptosis were found in the model group

　　Conclusion The experimental animal model can well simulate clinical manifestations such as ovarian function decline after clinical operation, and is an ideal animal model for studying complications after hysterectomy and screening drugs