动物造模,维吾尔医异常黑胆质证大鼠模型 z-bio 2022-11-04 215

　　Objective: To evaluate and discuss the biological markers of the rat model of abnormal black bile syndrome in Uyghur medicine by combining the observation of external characteristics with the study of urine metabolome

　　Methods: The animal model of rats was established with the theory of abnormal melancholia in Uygur medicine, and the external indicators such as hair, tongue, sleep, feces, mood, weight growth rate were observed and scored, and the urine was detected and analyzed with nuclear magnetic resonance hydrogen spectrum

　　Results: Compared with the control group, the external characteristics of the rats in the model group of abnormal black gallbladder syndrome changed significantly, including dry and hard feces, decreased urine volume and deepened color, dry fur, dark purple tongue coating with ecchymosis, and decreased weight growth rate; The content of 23 metabolites in urine metabolites was significantly reduced, including propionate, lactic acid, alanine, pyruvate, acetic acid, acetamide, glycoprotein, acetone, metformin, sarcosine, ornithine, glycine, creatine, creatinine, creatinine, creatinine β⁃ Galactose, uric acid ester, tyrosine, phenylalanine, hippuric acid, aminohippuric acid, formic acid and lysine, while urea, citric acid, allantoin and α⁃ The content of 4 metabolites such as ketoglutaric acid increased significantly

　　Conclusion: In the process of the occurrence and development of abnormal black gallbladder syndrome, the model group rats not only had obvious changes in external appearance, but also had obvious changes in internal multiple metabolic pathways. The obvious abnormality of urine metabolites may be related to the biological mechanism of abnormal black gallbladder syndrome.