动物造模,食蟹猴肌肉萎缩模型 z-bio 2022-11-04 357

　　Objective: To establish an animal model of muscle atrophy in cynomolgus monkeys by restricting lower limb activities, and to establish a method for model evaluation?

　　Methods: The right lower limb (ankle joint to knee joint) of cynomolgus monkey was fixed with macromolecule fiber bandage for 15 weeks, during which the weight was measured every two weeks? Bilateral calf circumference? Lower limb volume, modeling 11? 13? MRI examination of bilateral calf gastrocnemius was performed at 15 weeks, and muscle biopsy was performed at 15 weeks?

　　Results: After 15 weeks of modeling, there was no significant change in the weight of the animals? Right thigh circumference? The volume of right lower limb decreased significantly compared with that before modeling (P<0.05); Serum creatinine? Blood sugar? Alkaline phosphatase decreased significantly (P<0.05) and serum cholesterol level increased significantly (P<0.05); MRI showed atrophy of the right gastrocnemius muscle, and the volume of the right gastrocnemius muscle decreased compared with that before modeling; Muscle biopsy showed atrophy of right gastrocnemius muscle and thinning of muscle fibers; Reduction of cross sectional area of gastrocnemius fibers?

　　Conclusion: The muscle atrophy model was made by limiting the lower limb activity of cynomolgus monkey with external fixation. The modeling period was appropriate and the operability was strong? Comprehensive use of leg circumference? Lower limb drainage volume? Biochemical indicators? The evaluation method combining MRI imaging and muscle biopsy can be simple? Objective evaluation of the model of muscle atrophy in cynomolgus monkeys?