大气细颗粒物致炎动物模型,动物造模 z-bio 2022-11-04 312

　　Objective: To explore the pathogenic mechanism of air pollution on animals, and to establish an animal model of inflammation caused by air pollution by noninvasive tracheal instillation of PM2.5 suspension in BALB/c mice?

　　Methods: 150 SPF BALB/c mice were randomly divided into the blank control group? Normal saline group? PM2.5 low level group (2.5 mg/kg)? PM2.5 moderate group (5 mg/kg) and PM2.5 high group (10 mg/kg) were divided into 5 groups, and each dose group was given intratracheal instillation on the third day and the seventh day? Day 21? Day 35? On the 49th day, the tissue samples were taken 24 hours after the completion of the tracheal instillation operation, using ELISA? The method of HE staining of lung tissue pathology is used to verify the feasibility of non-invasive tracheal instillation method and whether the inflammation model is constructed successfully?

　　Results: The success rate of this modeling method was as high as 96%? With the method of tracheal instillation, the lung tissue inflammation score of the model mice was positively correlated with the prolongation of the airway instillation time and dose? After PM2.5 exposure, there is a large number of lymphocyte aggregation and macrophage infiltration of phagocytic particles in the lung, and the alveolar space is widened? Each exposure group was compared with the normal saline control group? Compared with the blank group, IL-6? TNF in lung homogenate- α The level increases, and the difference is most significant in the high-dose group?

　　Conclusion: In this experiment, the method of tracheal instillation was used to establish an inflammatory model in mice, which was successful and proved to be simple? It is reliable and can be widely used for repeated instillation of respiratory system in mice, which is conducive to further study of air pollution and other inflammatory mechanisms?