动物实验,阿尔茨海默氏病 z-bo 2020-08-22 445

　　Objective: Based on the establishment of experimental animal models of Alzheimer's disease in rats, to explore the pathological characteristics of brain tissue in amyloid cerebrovascular disease.

　　Method: In order to establish an animal model of Alzheimer's disease, Aβ1-42 fibers were injected into the hippocampus of rats under the guidance of a stereotaxic device. The water maze test is used to evaluate the advanced cognitive function and HE staining of rats. Congo red staining, α-actin and Aβ double-labeled immunofluorescence staining were used to observe the dynamic changes of amyloid deposits and amyloid cerebrovascular diseases in the rat brain.

　　Result: Compared with the fake operation control group, the model group's escape waiting time and the number of platform explorations are very different. HE staining showed that the hippocampus of model rats had decreased granular cells, cytopathic changes, glial cell proliferation and hippocampal small vessel wall degeneration. Congo red staining showed that Aβ fibers gradually deposited on the walls of small blood vessels, which thickened the walls and narrowed or occluded small blood vessels. Immunofluorescence staining showed that Aβ fibers in the brain tissue of the model group gradually migrated to small arteries.

　　Conclusion: Amyloid cerebrovascular disease is the main pathological change of Alzheimer's disease.