动物造模,子宫内膜异位症盆腔粘连大鼠模型 z-bio 2022-11-04 332

　　Objective: To explore the establishment method and evaluation index of endometriosis pelvic adhesion rat model.

　　Methods: Endometrium was transplanted into the mesentery of rats by autotransplantation. Eight rats were randomly selected at 1, 3, 5, 7, 14, 21 and 28 days after operation to observe the growth of the lesion. The degree of pelvic adhesions was evaluated by the American Reproductive Society Adhesion Scale (AFS) and Haber Adhesion Scale; At the same time, HE staining was performed on the focus and surrounding adherent peritoneum of each group; The tissue type plasminogen activator (tPA) content in the adherent tissue around the lesion was dynamically detected at 5,7,14,21,28 days after operation in the groups.

　　Results: The two adhesion scoring methods showed that typical pelvic adhesions had been formed 5 days after modeling; Compared with the blank control group and the sham operation group, the tPA content in the pelvic adhesion peritoneum of rats in each model group decreased extremely 5 days after modeling (P<0.01), and the tPA content increased 28 days after modeling, but it was still significantly lower than the blank control group and the sham operation group (P<0.01).

　　Conclusion: The model of pelvic adhesions of EMs was established by autologous transplantation of endometrium into mesentery. The model was mature 5 days after operation. There was a correlation between tPA and the occurrence of pelvic adhesions of EMs.