动物造模,高动力型感染性休克犬模型 z-bio 2022-11-10 286

　　Objective: To establish a canine model of hyperkinetic septic shock by intravenous injection of Escherichia coli.

　　Methods: 22 mongrel dogs were randomly divided into control group (n=11) and experimental group (n=11). After anaesthetized dogs, a double lumen central venous catheter was placed through the right femoral vein. The control group used a micro pump to pump normal saline into the canine femoral vein. The experimental group used the same method to pump Escherichia coli. PICCO catheter was placed through the right femoral artery. PICCO was used to monitor the systemic hemodynamics at various time points from 0 to 48 hours.

　　Results: Heart rate (HR), cardiac output (CO) and stroke volume (SV) in the experimental group increased significantly at 12 h (P<0.05), while systolic blood pressure (SBP) and peripheral vascular resistance (SVR) decreased significantly at 12 h (P<0.05), while="" other="" hemodynamic="" indicators="" such="" as="" central="" venous="" pressure="" pulmonary="" artery="" wedge="" oxygen="" delivery="" consumption="" and="" uptake="" rate="" had="" no="" significant="" difference="" p="">0.05). Significant decrease in urine volume was observed in the experimental group from 12 hours; There were significant differences in peak systolic blood flow velocity (PSV) and resistance index (RI) between the two groups (P<0.05).

　　Conclusion: The canine model of hyperkinetic septic shock can be successfully prepared by pumping live E. coli into the canine femoral vein with a micro pump, which can provide an experimental basis for the study of septic shock.