动物造模,2型糖尿病大鼠模型 z-bio 2022-11-10 267

　　Objective: To establish and analyze the characteristics of plasma metabonomics in type 2 diabetes (T2DM) rats with Uygur medicine abnormal black gallbladder syndrome.

　　Methods: The T2DM Uyghur medicine abnormal black bile syndrome rat model was established and plasma samples were collected to test the nuclear magnetic resonance hydrogen spectrum, and the data were analyzed by multivariate analysis.

　　Results: The contents of glucose and lactic acid in plasma of rats with simple abnormal black bile syndrome decreased, while the contents of VLDL, LDL and carnitine increased significantly (P<0.05); T2DM Uighur medicine abnormal black bile syndrome group rats with multiple amino acids (alanine, isoleucine, valine, leucine, tyrosine, glutamic acid, phenylalanine, methyl histidine, glycine), lactic acid, pyruvate, glycoprotein, citric acid, creatinine β- The contents of hydroxybutyric acid, acetoacetic acid, acetic acid, creatine, carnitine, squaroositol, VLDL and LDL decreased, while the contents of glucose and taurine increased significantly (P<0.05).

　　Conclusion: The protein metabolism, phenylalanine and tyrosine metabolism pathway, glycolysis pathway and lipid metabolism pathway have obvious changes in T2DM Uighur medicine abnormal black bile syndrome model rats; The changes of plasma metabonomics, to some extent, reflect the pathological changes in the T2DM rat model of Uighur medicine with abnormal black gallbladder syndrome.