动物造模,皮下移植瘤模型 z-bio 2022-11-10 329

　　Objective: To explore the differences of subcutaneous transplantation of lung cancer in different parts of the body, and to provide a basis for lung cancer researchers.

　　Methods: Lewis lung adenocarcinoma cells (ll2-luc-m38) stably expressing luciferase were injected subcutaneously into the right armpit, the right groin and the foot pad of C57 BL/6 mice, respectively. The tumor formation and metastasis of mice transplanted subcutaneously were regularly observed with small animal imaging system, and the survival time and mortality of tumor bearing mice in different parts were observed. The tumor tissue was obtained by paraffin embedding, sectioning Pathological diagnosis was made by HE staining. The lung tissues were fixed and the metastatic foci were observed immediately after the mice were killed. The survival and metastasis of the transplanted tumor were observed.

　　Results: The axillary group and groin formed tumors earlier, with a tumor formation rate of 100%, and the foot pad formed tumors later, with a tumor formation rate of 33%; The tumor volume in groin group and axillary group increased rapidly, and the tumor volume in groin group increased fastest; On the 21st day after inoculation, 70% of the mice in the axillary group had lung metastasis, with a large number of metastatic foci; 50% of the mice in the groin group had pulmonary metastasis, and the number of metastatic foci was small; No lung metastasis was observed in the footrest group. The death rate of the footrest group was the highest. The mice in the inguinal group and the subaxillary group could be transplanted subcutaneously for tumor resection, and the survival rate was 100%.

　　Conclusion: in the subcutaneous transplantation tumor model of lewis lung cancer in mice, the inguinal group and the axillary group have a high tumor formation rate, can tolerate tumor resection, low risk of death, easy to monitor, simple operation and high repeatability, and the axillary group has better metastasis.