动物造模,肠系膜上静脉血栓形成大鼠模型 z-bio 2022-11-11 421

　　Objective: To establish a rat model of superior mesenteric vein thrombosis (SMVT) by ligation and simulate the pathological process of the disease, so as to provide basic preparation for the study of its pathogenesis and treatment.

　　Methods: 96 male SD rats were randomly divided into three groups: group A (sham operation group), group B (strangulated type group) and group C (simple type group), with 32 rats in each group. The rats in group A only opened the abdominal cavity without blocking the blood supply, and the rats were killed in batches at 8,24,48,72 h after operation; Rats in group B and group C were ligated to establish strangulated and simple models of superior mesenteric vein thrombosis respectively. The rats were killed in batches at 8,24,48,72 h after modeling. HE staining was used to observe the changes of intestinal histomorphology and pathological score of the injury; Determination of serum intestinal fatty acid binding protein (IFABP) and α- Glutathione S-transferase（ α- GST) level.

　　Results: The results of HE staining and pathological score showed that compared with rats in group A, the intestinal tissues of rats in group B and group C showed different degrees of blood stasis and injury, which were gradually aggravated in group B and gradually relieved in group C. The degree of blood stasis and injury was positively related to the extent of ligation. The results of serum ELISA showed that the IFABP and α- The level of GST increased in different degrees (P<0.05), and the increase was positively correlated with the extent of ligation.

　　Conclusion: In this study, the rat model of superior mesenteric vein thrombosis was successfully established by ligation, which is simple and easy to operate with high success rate, and can be used in related research.