动物造模 z-bio 2022-11-11 264

　　Objective: To observe the function of dendritic cells derived from peripheral blood of white haired black eyed rabbits and Japanese white rabbits with allergic rhinitis induced by ovalbumin, and to reveal the possible mechanism of WHBE rabbits' sensitivity to AR.

　　Methods: The AR models of WHBE rabbits and JW rabbits were induced by OVA. After each challenge, the general signs of the animals were observed, the nasal mucosa was stained with HE, and the histopathological changes were observed. The peripheral blood DC was isolated, and the expression of CD86 and the ability of DC to absorb antigen were detected by flow cytometry. At the same time, the mRNA expression of mannose receptor (MR) in peripheral blood DC was detected by fluorescent quantitative PCR. T cells were further labeled with CFSE, and the proliferation of T cells induced by peripheral blood DC was detected by flow cytometry.

　　Results: The observation of general signs and HE staining showed that both the WHBE rabbit and JW rabbit model groups showed typical allergic rhinitis symptoms and histopathological changes, indicating that the model was successfully constructed. The expression of DC CD86 in peripheral blood of WHBE rabbit AR model group was not only significantly higher than that of normal control group, but also significantly higher than that of JW rabbit AR model group (P<0.01, P<0.01). Under normal steady state, the relative mRNA expression of peripheral blood DC MR in WHBE rabbits was significantly higher than that in JW rabbits (P<0.01). After OVA induced AR model, the mRNA expression level of peripheral blood DC MR in WHBE rabbits was significantly higher than that in JW rabbits (P<0.05). Moreover, the ability of peripheral blood DC to absorb OVA647 in the WHBE rabbit model group was significantly higher than that in the normal control group (P<0.01), and also significantly higher than that in the JW rabbit model group (P<0.01).

　　Conclusion: WHBE rabbit DC is more sensitive to allergens, which may be related to its higher expression of antigen recognition receptor MR and stronger ability of differentiation, maturation and antigen uptake.