动物造模,胶质瘤原位移植瘤模型 z-bio 2022-11-21 321

　　Objective: To establish a stable and real-time nude mouse model of glioma orthotopic transplantation.

　　Methods: U251 glioma cells were infected with lentivirus carrying luciferase Luc and green fluorescent protein GFP genes. The cell lines stably expressing GFP Luc fluorescence were screened by flow cytometry. CCK-8 test, cell cycle test, Transfer tumor migration and invasion test were used to evaluate whether the proliferation, migration and invasion of fluorescent cells were changed; The cells were inoculated into the caudate nucleus of the brain of nude mice to establish a glioma orthotopic transplantation tumor model. The growth of brain tumors was monitored using the mouse in vivo imaging system. The pathological characteristics and tumorigenicity of cells in the brain of nude mice were evaluated by paraffin sections and HE staining.

　　Results: U251 glioma cell line and animal model stably expressing GFP fluorescence and luciferase fluorescence were successfully constructed, and lentivirus integration did not change cell proliferation, migration and invasion; The growth cycle of the model was moderate, the tumor formation rate was high, the tumor grew stably in the brain, and the HE section was consistent with the characteristics of human glioma.

　　Conclusion: Compared with the traditional cells, the double fluorescent labeled glioma cells are more beneficial to the experimental study of glioma animal models; The nude mouse model of U251 GFP Luc glioma cells has similar tumor growth and pathological characteristics with human glioma, and can observe tumor growth in real time, which can be used as an ideal animal model for glioma experimental research.