动物造模 z-bio 2022-11-21 431

　　Objective: To observe the difference of short wave optoprotein (S-opsin) expression between stroboscopic induced myopia and form deprivation myopia in guinea pigs, and to explore the causes.

　　Methods: Thirty six normal 2-week old guinea pigs were randomly divided into three groups: stroboscopic group (FLM group, n=13), form deprivation group (FDM group, n=12), and control group (n=11). In FLM group, the feeding cage is equipped with stroboscope (frequency 0.5 Hz), and the cage is equipped with LED; The right eye of the guinea pigs in FDM group was covered with a translucent eye mask to ensure the normal movement of the eyelids of the guinea pigs; Guinea pigs in the control group were not specially treated. The diopter, axial length and corneal curvature radius of the right eye of guinea pigs were measured on the first day (week 0) and the sixth week of modeling, and the expression of S-opsin was observed by immunofluorescence.

　　Results: At week 0, there was no significant difference in diopter, axial length and radius of corneal curvature between FLM, FDM and control groups (P>0.05). After 6 weeks of modeling, compared with the control group, FLM group and FDM group had significant differences in the changes of diopter and axial length (P<0.05), while="" there="" was="" no="" significant="" difference="" in="" the="" changes="" of="" corneal="" radius="" curvature="" p="" indicating="" that="" myopia="" model="" successfully="" established.="" between="" flm="" group="" and="" fdm="" diopter="" axial="" length="" change="">0.05). The results of immunofluorescence showed that the gray value of visual protein in FLM group was higher than that in control group and higher than that in FDM group. The difference between any two groups was significant (P<0.001).

　　Conclusion: Both stroboscopic flash and form deprivation can establish myopia models. The production of S-opsin increases in stroboscopic induced myopia models, while the production of S-opsin decreases in form deprivation myopia models, indicating that the mechanisms of the two myopia models may be different.