动物造模,急性肝功能衰竭模型 z-bio 2022-11-21 326

　　Objective: To explore the effect of different proportions of hepatectomy volume on acute liver failure in Bama miniature pigs, and to provide a suitable method for establishing an appropriate model of acute liver failure after major hepatectomy in miniature pigs.

　　Methods: 75%, 85% and 95% hepatectomy were performed respectively. The residual liver was examined by CT and the survival was recorded. Blood was drawn regularly before operation, 1, 3, 5 days after operation and 1, 2 and 3 weeks after operation to detect the liver function, obtain HE staining of liver tissue, and examine the liver pathology.

　　Results: The average survival time of 75%, 85% and 95% hepatectomy minipigs was (19.0 ± 5.6) days, (17.3 ± 5.5) days and (1.3 ± 1.5) days. The pathological scores of Bama minipigs with different hepatectomy ratios were (5.67 ± 0.52), (8.17 ± 0.82) and (8.50 ± 0.71) respectively. With the increase of the proportion of hepatectomy, the incidence rate of liver failure increased. 85% hepatectomy can significantly increase the levels of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, alkaline phosphatase, lactate dehydrogenase and total bile acid.

　　Conclusion: 85% volume hepatectomy can create a typical miniature pig model of acute liver failure.