动物实验,肠道菌群 z-bo 2020-08-22 502

　　Objective: To investigate the effect of dexamethasone pollution in water environment on the intestinal flora of mice.

　　Methods: Twenty Balb/c mice were randomly divided into a control group and an experimental group, 5 in each group. The drinking water received by the experimental low-dose group contained 0.035g dexamethasone sodium phosphate, the medium-dose group was 0.225g, and the high-dose group The group was 2.25 g, and the control group was drinking water without dexamethasone sodium phosphate. Observe the mouse movement, fur, feces, etc. changes every day. On the 36th day of perfusion, the mice were sacrificed, and blind tissues were collected to extract bacterial genomic DNA, amplify the variable region of 16SDNAV6, and analyze the amplified products after denaturing gel gradient electrophoresis (DGGE). Cut off, amplify and purify the main bands on the DGGE map, clone and sequence, and perform BLAST alignment and analysis of the sequence.

　　Results: The mice in each experimental group showed irritability, fighting and tail biting. The cluster analysis of the DGGE chart shows that the ileocecal area of each group of mice has a relatively stable flora. Principal component analysis shows that the main flora of each group is different. Compared with the control group, the low-dose group's flora diversity analysis significantly increased the number and type of flora (P\u003c0.05) and it has increased significantly (P\u003c0.01). The sequencing analysis of the 16SDNAV6 region showed that there were 15 common bacterial genera and 2 different bacterial genera in the experimental group and the control group, and the main bacterial species and proportions were different. Lactic acid bacteria were colonized in the control group, but the lactic acid bacteria disappeared in the medium and high dose experimental groups, while Shigella appeared.

　　Conclusion: Drinking water pollution can affect the nervous system of mice, change the type and number of bacteria in the mouse intestine, change the proportion of main bacteria, increase the diversity of the flora and inhibit the colonization of internal probiotics in the intestine. Promote the invasion of intestinal pathogens.