动物造模,兔低血糖模型 z-bio 2022-11-21 306

　　Objective: To establish a rabbit hypoglycemia model and evaluate the accuracy and timeliness of subcutaneous continuous glucose monitoring system (CGMS) in hypoglycemia monitoring.

　　Methods: 16 female New Zealand white rabbits were randomly divided into 4 groups with 4 rabbits in each group. The control group received continuous intravenous injection of normal saline, and the experimental group animals received continuous intravenous injection of insulin. According to different doses, they were divided into insulin 0.1 U/(kg · h) group (RI=0.1 U group), insulin 0.2 U/(kg · h) group (RI=0.2 U group), and insulin 0.4 U/(kg · h) group (RI=0.4 U group). During the test, CGMS was monitored for 240 min, and ear vein blood was collected at an interval of 30 min, and blood glucose (BG monitoring value) was monitored by a hand-held blood glucose meter.

　　Results: During the study period, 1296 CGMS monitoring data were obtained, and 136 BG monitoring data matched with CGMS time. Both BG and CGMS decreased significantly after insulin administration, and the reduction rates of BG and CGMS in RI=0.1U group were 0.016 and 0.017 mmol/L per minute, respectively; RI=0.2U: 0.04 and 0.027 mmol/L per minute, respectively; RI=0.4 0.049 and 0.032 mmol/L in group U, respectively. BG-CGMS matching data are classified into hypoglycemia and normal blood glucose according to whether the BG monitoring value is lower than 4.4 mmol/L. During hypoglycemia, the average deviation of BG-CGMS was 0.55 mmol/L (upper and lower limit: -0.98~2.08 mmol/L), and the percentage of absolute difference (RAD) was 40.2% ± 45.2%; The average deviation of BG-CGMS in normal blood glucose was -0.19 mmol/L (upper and lower limit: -1.38~1.00 mmol/L), and the RAD was 5.8% ± 5.3%. Error grid analysis (EGA) showed that area A accounted for 93.4%, area B 0.7% and area D 5.9%, and that area D was distributed in areas with low BG and high CGMS.

　　Conclusion: The results of this study suggest that when the blood glucose decreases rapidly, CGMS appears obvious hysteresis, and when the blood glucose decreases below 4.4 mmol/L, CGMS has the risk of overestimating blood glucose. This risk should be fully considered in the clinical application of CGMS.