动物造模,阿尔茨海默病模型 z-bio 2022-11-21 324

　　Purpose: Different concentrations of amyloid A β After injection of 25-35 lateral ventricles, observe the changes of learning and memory ability of Morris water maze in rats, and explore the A β 25-35 is the best concentration for injection.

　　Methods: Male SD rats were randomly divided into sham operation group and model group. Model group A β 25-35 injection concentration is 2, 4 and 8 respectively μ g/ μ L。 According to the Stereotactic Map of Rat Brain, select the right lateral ventricle to inject aggregated A β 25-35, prepare AD rat model. Morris water maze was used to test the changes of learning and memory ability of rats in each group 7 days after the model was successfully established.

　　Results: Compared with the average swimming speed, there was no significant difference among the groups (P>0.05). The escape latency results showed that compared with the sham operation group, the escape latency time of the model group rats was significantly increased (P<0.05); In model group, with injection 2 μ g/ μ Comparison of rats with L, injection 4 μ g/ μ L and 8 μ g/ μ The escape latency time of rats increased significantly after L (P<0.05); 8="" four="" l="" and="" there="" was="" no="" significant="" difference="" between="" groups="" p="">0.05). The activity time and distance of the target quadrant were displayed. Compared with the sham operation group, the model group was injected with different doses of A β The target quadrant activity time and distance of 25-35 rats were significantly reduced (P<0.05), but="" there="" was="" no="" significant="" difference="" between="" different="" concentrations="" of="" the="" model="" group="" p="">0.05). The results of space exploration showed that compared with the sham operation group, the model group was injected with different doses of A β The times of 25-35 rats crossing the platform were significantly reduced (P<0.05); In model group, with injection 2 μ g/ μ Comparison of rats with L, injection 4 μ g/ μ L and 8 μ g/ μ After L, the number of rats passing through the platform decreased significantly (P<0.05). 8="" four="" l="" and="" there="" was="" no="" significant="" difference="" between="" groups="" p="">0.05).

　　Conclusion: A was injected into unilateral ventricle β When preparing AD model of rats with 25-35 protein, inject A β The recommended concentration of 25-35 is 4 μ g/ μ L。