动物造模,高脂血症模型 z-bio 2022-11-22 333

　　Objective: To establish hyperlipidemia in SD rats fed with high-fat diet for 8 weeks, verify the feasibility of this modeling method, and explore the regularity of blood lipid changes in rats at different time points.

　　Methods: 30 normal male 8-week old SD rats were randomly divided into control group, model group 1 and model group 2 according to their weight after adaptive feeding for 1 week, with 10 rats in each group. The control group was fed with normal diet, while the model group 2 was fed with high-fat and high cholesterol diet for 8 weeks. The diet and drinking water of each group were measured daily, and the padding was replaced and weighed 4 days later. At the end of the 4th, 6th and 8th week, the animals fasted overnight and collected blood from the orbital venous plexus to measure the four levels of blood lipids in each group. The animals were killed at the end of 8 weeks, and the liver and aorta of rats in each group were collected for HE staining.

　　Results: Compared with the control group, the daily average diet of model 1 group increased, but the difference was not statistically significant; The daily average diet of model group 2 was significantly reduced (P<0.01). Compared with the control group, the daily water consumption of the model group and the control group decreased significantly (P<0.01). Compared with the control group, the liver/body mass index of the model group and the control group increased significantly (P<0.01). Compared with the control group, the final weight of model group 2 increased, but only model group 1 had statistical significance (P<0.05). In terms of blood lipid, compared with the control group, at the end of 4 and 6 weeks, the level of TC in model group 2 was significantly higher, with a statistically significant difference (P<0.01 or P<0.05); at the end of 8 weeks, the level of LDL-c in model group 1 was significantly higher, and the level of HDL-c was lower, with a statistically significant difference (P<0.05). HE staining of liver showed that hepatocytes in the control group were arranged normally and stained evenly, while hepatocytes in the model group 2 had large area of steatosis, and a few liver sinuses were congested and inflammatory cells infiltrated. HE staining of the aorta showed that the structure of the aorta in the three groups was normal.

　　Conclusion: SD rats fed with high-fat diet can establish hyperlipidemia, and the model is hypercholesterolemia with severe fatty liver. At the same time, in the process of hyperlipidemia experiment in SD rats fed with high-fat and high cholesterol diet, the blood lipid fluctuation showed a state of increase - internal adaptation - increase. In the process of modeling, how to overcome the problem of animal anorexia and internal regulation of cholesterol metabolism is the key to modeling with high-fat feed.