动物造模,胃癌原位移植模型 z-bio 2022-11-22 309

　　Objective: To study the effect of heptamethine cyanine near-infrared fluorescence (NIRF) dye in vivo imaging of orthotopic gastric cancer transplantation model.

　　Methods: Human gastric cancer cell line HepG2 labeled with fluorescein was transplanted into nude mice in situ to establish tumor model and induce gastric ulcer model; Bioluminescence imaging and NIRF imaging were used to observe the absorption of near-infrared fluorescent dye in gastric cancer tissues; To explore the effects of hypoxia and anion transfer peptide (OATP) on the absorption of NIRF dye in gastric cancer tissues, and to determine the specificity of NIRF dye targeting tumor cells.

　　Results: There was a good correlation between NIRF signal and bioluminescence signal in vivo imaging of gastric cancer orthotopic transplantation model. Strong NIRF fluorescence signal can be obtained in gastric cancer tissue, but no fluorescence signal can be detected in gastric ulcer. Hypoxia can enhance the absorption of NIRF dye by gastric cancer cells, while the anion transport peptide specific inhibitor, sodium sulfbromphthalein (BSP), can significantly reduce the absorption of NIRF dye by tumor cells.

　　Conclusion: Heptamethine cyanine near-infrared fluorescent dye can target the orthotopic transplantation model of gastric cancer.