动物造模,金黄色葡萄球菌关节炎小鼠模型 z-bio 2022-11-22 343

　　Objective: To establish and analyze the arthritis model infected by conditionally infected Staphylococcus aureus, so as to provide an animal model for the research of arthritis mechanism and drug development.

　　Methods: The mice were immunosuppressed with cyclophosphamide, and then inoculated with Staphylococcus aureus through the tail vein. The model was evaluated by observing the joint appearance characteristics, arthritis index analysis and pathological observation scores.

　　Results: From the first day after inoculation, the joints of the mice were slightly swollen, and the pathological changes of the joint tissues developed from a small amount of synovial hyperplasia with disordered arrangement and evacuation to synovial thickening, extending into the joint cavity and increasing neutrophil infiltration.

　　Conclusion: The successful establishment of conditional infection SA model can simulate the clinical symptoms and course of human arthritis in a short time, and reflect the pathogenesis of human arthritis in terms of infection and immune mechanism.